anti ip3r Search Results


anti rabbit pip3r  (Boster Bio)
91
Boster Bio anti rabbit pip3r
Anti Rabbit Pip3r, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
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anti-ip 3 r-iii  (Becton Dickinson)
90
Becton Dickinson anti-ip 3 r-iii
The expression of several cGMP/PKGI signaling proteins in colons from IRAG1-KO (KO) and IRAG1-WT (WT) mice. Protein expression of IRAG1-KO mice and their WT littermates (respective age: 15 weeks) were analyzed by western blot ( A – F ) and normalized to total protein (TP) for quantification ( G ). ( A ) Representative Western blot of IRAG1 in colons of male and female IRAG1-WT (total: n = 18; male: n = 10; female: n = 8) showed no differences between the sexes and no IRAG1 protein was found in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11). ( B ) Western blot of IP 3 R-I showed a significant decrease in its expression in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared to WT littermates (total: n = 21; male: n = 11; female: n = 10). ( C ) The tremendous reduction of PKGIβ protein in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared to IRAG1-WT mice (total: n = 24; male: n = 12; female: n = 12). ( D ) PKGIα expression was not altered between IRAG1-KO (total: n = 20; male: n = 10; female: n = 10) and IRAG1-WT mice (total: n = 22; male: n = 11; female: n = 11). ( E ) Reduced IP 3 R-III expression in IRAG1-KO mice (total: n = 21; male: n = 10; female: n = 11) in relation to IRAG1-WT mice (total: n = 17; male: n = 9; female: n = 8). ( F ) No altered NO-GC-β1 expression between IRAG1-KO (total: n = 22; male: n = 11; female: n = 11) and IRAG1-WT mice (total: n = 23; male: n = 12; female: n = 11). ( G ) Statistical analysis of the expression of investigated proteins ( A – F ). The mean ± SEM is shown in the graphs, and significant differences are shown by (*) ( p < 0.05), (**) ( p < 0.01) and (***) ( p < 0.001). Images of total protein (TP) were shown in .
Anti Ip 3 R Iii, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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anti-ip3r antibody  (Merck KGaA)
90
Merck KGaA anti-ip3r antibody
The expression of several cGMP/PKGI signaling proteins in colons from IRAG1-KO (KO) and IRAG1-WT (WT) mice. Protein expression of IRAG1-KO mice and their WT littermates (respective age: 15 weeks) were analyzed by western blot ( A – F ) and normalized to total protein (TP) for quantification ( G ). ( A ) Representative Western blot of IRAG1 in colons of male and female IRAG1-WT (total: n = 18; male: n = 10; female: n = 8) showed no differences between the sexes and no IRAG1 protein was found in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11). ( B ) Western blot of IP 3 R-I showed a significant decrease in its expression in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared to WT littermates (total: n = 21; male: n = 11; female: n = 10). ( C ) The tremendous reduction of PKGIβ protein in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared to IRAG1-WT mice (total: n = 24; male: n = 12; female: n = 12). ( D ) PKGIα expression was not altered between IRAG1-KO (total: n = 20; male: n = 10; female: n = 10) and IRAG1-WT mice (total: n = 22; male: n = 11; female: n = 11). ( E ) Reduced IP 3 R-III expression in IRAG1-KO mice (total: n = 21; male: n = 10; female: n = 11) in relation to IRAG1-WT mice (total: n = 17; male: n = 9; female: n = 8). ( F ) No altered NO-GC-β1 expression between IRAG1-KO (total: n = 22; male: n = 11; female: n = 11) and IRAG1-WT mice (total: n = 23; male: n = 12; female: n = 11). ( G ) Statistical analysis of the expression of investigated proteins ( A – F ). The mean ± SEM is shown in the graphs, and significant differences are shown by (*) ( p < 0.05), (**) ( p < 0.01) and (***) ( p < 0.001). Images of total protein (TP) were shown in .
Anti Ip3r Antibody, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-ip3r antibody/product/Merck KGaA
Average 90 stars, based on 1 article reviews
anti-ip3r antibody - by Bioz Stars, 2026-02
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mouse anti-ip3r  (NeuroMab)
90
NeuroMab mouse anti-ip3r
The expression of several cGMP/PKGI signaling proteins in colons from IRAG1-KO (KO) and IRAG1-WT (WT) mice. Protein expression of IRAG1-KO mice and their WT littermates (respective age: 15 weeks) were analyzed by western blot ( A – F ) and normalized to total protein (TP) for quantification ( G ). ( A ) Representative Western blot of IRAG1 in colons of male and female IRAG1-WT (total: n = 18; male: n = 10; female: n = 8) showed no differences between the sexes and no IRAG1 protein was found in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11). ( B ) Western blot of IP 3 R-I showed a significant decrease in its expression in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared to WT littermates (total: n = 21; male: n = 11; female: n = 10). ( C ) The tremendous reduction of PKGIβ protein in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared to IRAG1-WT mice (total: n = 24; male: n = 12; female: n = 12). ( D ) PKGIα expression was not altered between IRAG1-KO (total: n = 20; male: n = 10; female: n = 10) and IRAG1-WT mice (total: n = 22; male: n = 11; female: n = 11). ( E ) Reduced IP 3 R-III expression in IRAG1-KO mice (total: n = 21; male: n = 10; female: n = 11) in relation to IRAG1-WT mice (total: n = 17; male: n = 9; female: n = 8). ( F ) No altered NO-GC-β1 expression between IRAG1-KO (total: n = 22; male: n = 11; female: n = 11) and IRAG1-WT mice (total: n = 23; male: n = 12; female: n = 11). ( G ) Statistical analysis of the expression of investigated proteins ( A – F ). The mean ± SEM is shown in the graphs, and significant differences are shown by (*) ( p < 0.05), (**) ( p < 0.01) and (***) ( p < 0.001). Images of total protein (TP) were shown in .
Mouse Anti Ip3r, supplied by NeuroMab, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-ip3r/product/NeuroMab
Average 90 stars, based on 1 article reviews
mouse anti-ip3r - by Bioz Stars, 2026-02
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anti-ip3r-2 antibody (epitope: npdyrdaqnegknv)  (GenScript corporation)
90
GenScript corporation anti-ip3r-2 antibody (epitope: npdyrdaqnegknv)
The expression of several cGMP/PKGI signaling proteins in colons from IRAG1-KO (KO) and IRAG1-WT (WT) mice. Protein expression of IRAG1-KO mice and their WT littermates (respective age: 15 weeks) were analyzed by western blot ( A – F ) and normalized to total protein (TP) for quantification ( G ). ( A ) Representative Western blot of IRAG1 in colons of male and female IRAG1-WT (total: n = 18; male: n = 10; female: n = 8) showed no differences between the sexes and no IRAG1 protein was found in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11). ( B ) Western blot of IP 3 R-I showed a significant decrease in its expression in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared to WT littermates (total: n = 21; male: n = 11; female: n = 10). ( C ) The tremendous reduction of PKGIβ protein in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared to IRAG1-WT mice (total: n = 24; male: n = 12; female: n = 12). ( D ) PKGIα expression was not altered between IRAG1-KO (total: n = 20; male: n = 10; female: n = 10) and IRAG1-WT mice (total: n = 22; male: n = 11; female: n = 11). ( E ) Reduced IP 3 R-III expression in IRAG1-KO mice (total: n = 21; male: n = 10; female: n = 11) in relation to IRAG1-WT mice (total: n = 17; male: n = 9; female: n = 8). ( F ) No altered NO-GC-β1 expression between IRAG1-KO (total: n = 22; male: n = 11; female: n = 11) and IRAG1-WT mice (total: n = 23; male: n = 12; female: n = 11). ( G ) Statistical analysis of the expression of investigated proteins ( A – F ). The mean ± SEM is shown in the graphs, and significant differences are shown by (*) ( p < 0.05), (**) ( p < 0.01) and (***) ( p < 0.001). Images of total protein (TP) were shown in .
Anti Ip3r 2 Antibody (Epitope: Npdyrdaqnegknv), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-ip3r-2 antibody (epitope: npdyrdaqnegknv)/product/GenScript corporation
Average 90 stars, based on 1 article reviews
anti-ip3r-2 antibody (epitope: npdyrdaqnegknv) - by Bioz Stars, 2026-02
90/100 stars
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antibodies against ip 3 r 07–1210  (Merck KGaA)
90
Merck KGaA antibodies against ip 3 r 07–1210
The expression of several cGMP/PKGI signaling proteins in colons from IRAG1-KO (KO) and IRAG1-WT (WT) mice. Protein expression of IRAG1-KO mice and their WT littermates (respective age: 15 weeks) were analyzed by western blot ( A – F ) and normalized to total protein (TP) for quantification ( G ). ( A ) Representative Western blot of IRAG1 in colons of male and female IRAG1-WT (total: n = 18; male: n = 10; female: n = 8) showed no differences between the sexes and no IRAG1 protein was found in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11). ( B ) Western blot of IP 3 R-I showed a significant decrease in its expression in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared to WT littermates (total: n = 21; male: n = 11; female: n = 10). ( C ) The tremendous reduction of PKGIβ protein in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared to IRAG1-WT mice (total: n = 24; male: n = 12; female: n = 12). ( D ) PKGIα expression was not altered between IRAG1-KO (total: n = 20; male: n = 10; female: n = 10) and IRAG1-WT mice (total: n = 22; male: n = 11; female: n = 11). ( E ) Reduced IP 3 R-III expression in IRAG1-KO mice (total: n = 21; male: n = 10; female: n = 11) in relation to IRAG1-WT mice (total: n = 17; male: n = 9; female: n = 8). ( F ) No altered NO-GC-β1 expression between IRAG1-KO (total: n = 22; male: n = 11; female: n = 11) and IRAG1-WT mice (total: n = 23; male: n = 12; female: n = 11). ( G ) Statistical analysis of the expression of investigated proteins ( A – F ). The mean ± SEM is shown in the graphs, and significant differences are shown by (*) ( p < 0.05), (**) ( p < 0.01) and (***) ( p < 0.001). Images of total protein (TP) were shown in .
Antibodies Against Ip 3 R 07–1210, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies against ip 3 r 07–1210/product/Merck KGaA
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antibodies against ip 3 r 07–1210 - by Bioz Stars, 2026-02
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anti-ip3r (pa5907)  (Abmart Inc)
90
Abmart Inc anti-ip3r (pa5907)
The expression of several cGMP/PKGI signaling proteins in colons from IRAG1-KO (KO) and IRAG1-WT (WT) mice. Protein expression of IRAG1-KO mice and their WT littermates (respective age: 15 weeks) were analyzed by western blot ( A – F ) and normalized to total protein (TP) for quantification ( G ). ( A ) Representative Western blot of IRAG1 in colons of male and female IRAG1-WT (total: n = 18; male: n = 10; female: n = 8) showed no differences between the sexes and no IRAG1 protein was found in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11). ( B ) Western blot of IP 3 R-I showed a significant decrease in its expression in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared to WT littermates (total: n = 21; male: n = 11; female: n = 10). ( C ) The tremendous reduction of PKGIβ protein in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared to IRAG1-WT mice (total: n = 24; male: n = 12; female: n = 12). ( D ) PKGIα expression was not altered between IRAG1-KO (total: n = 20; male: n = 10; female: n = 10) and IRAG1-WT mice (total: n = 22; male: n = 11; female: n = 11). ( E ) Reduced IP 3 R-III expression in IRAG1-KO mice (total: n = 21; male: n = 10; female: n = 11) in relation to IRAG1-WT mice (total: n = 17; male: n = 9; female: n = 8). ( F ) No altered NO-GC-β1 expression between IRAG1-KO (total: n = 22; male: n = 11; female: n = 11) and IRAG1-WT mice (total: n = 23; male: n = 12; female: n = 11). ( G ) Statistical analysis of the expression of investigated proteins ( A – F ). The mean ± SEM is shown in the graphs, and significant differences are shown by (*) ( p < 0.05), (**) ( p < 0.01) and (***) ( p < 0.001). Images of total protein (TP) were shown in .
Anti Ip3r (Pa5907), supplied by Abmart Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-ip3r (pa5907)/product/Abmart Inc
Average 90 stars, based on 1 article reviews
anti-ip3r (pa5907) - by Bioz Stars, 2026-02
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polyclonal anti-ip3r rr1790x  (American Qualex)
90
American Qualex polyclonal anti-ip3r rr1790x
The expression of several cGMP/PKGI signaling proteins in colons from IRAG1-KO (KO) and IRAG1-WT (WT) mice. Protein expression of IRAG1-KO mice and their WT littermates (respective age: 15 weeks) were analyzed by western blot ( A – F ) and normalized to total protein (TP) for quantification ( G ). ( A ) Representative Western blot of IRAG1 in colons of male and female IRAG1-WT (total: n = 18; male: n = 10; female: n = 8) showed no differences between the sexes and no IRAG1 protein was found in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11). ( B ) Western blot of IP 3 R-I showed a significant decrease in its expression in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared to WT littermates (total: n = 21; male: n = 11; female: n = 10). ( C ) The tremendous reduction of PKGIβ protein in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared to IRAG1-WT mice (total: n = 24; male: n = 12; female: n = 12). ( D ) PKGIα expression was not altered between IRAG1-KO (total: n = 20; male: n = 10; female: n = 10) and IRAG1-WT mice (total: n = 22; male: n = 11; female: n = 11). ( E ) Reduced IP 3 R-III expression in IRAG1-KO mice (total: n = 21; male: n = 10; female: n = 11) in relation to IRAG1-WT mice (total: n = 17; male: n = 9; female: n = 8). ( F ) No altered NO-GC-β1 expression between IRAG1-KO (total: n = 22; male: n = 11; female: n = 11) and IRAG1-WT mice (total: n = 23; male: n = 12; female: n = 11). ( G ) Statistical analysis of the expression of investigated proteins ( A – F ). The mean ± SEM is shown in the graphs, and significant differences are shown by (*) ( p < 0.05), (**) ( p < 0.01) and (***) ( p < 0.001). Images of total protein (TP) were shown in .
Polyclonal Anti Ip3r Rr1790x, supplied by American Qualex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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anti-ip3r  (Wanleibio)
90
Wanleibio anti-ip3r
The expression of several cGMP/PKGI signaling proteins in colons from IRAG1-KO (KO) and IRAG1-WT (WT) mice. Protein expression of IRAG1-KO mice and their WT littermates (respective age: 15 weeks) were analyzed by western blot ( A – F ) and normalized to total protein (TP) for quantification ( G ). ( A ) Representative Western blot of IRAG1 in colons of male and female IRAG1-WT (total: n = 18; male: n = 10; female: n = 8) showed no differences between the sexes and no IRAG1 protein was found in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11). ( B ) Western blot of IP 3 R-I showed a significant decrease in its expression in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared to WT littermates (total: n = 21; male: n = 11; female: n = 10). ( C ) The tremendous reduction of PKGIβ protein in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared to IRAG1-WT mice (total: n = 24; male: n = 12; female: n = 12). ( D ) PKGIα expression was not altered between IRAG1-KO (total: n = 20; male: n = 10; female: n = 10) and IRAG1-WT mice (total: n = 22; male: n = 11; female: n = 11). ( E ) Reduced IP 3 R-III expression in IRAG1-KO mice (total: n = 21; male: n = 10; female: n = 11) in relation to IRAG1-WT mice (total: n = 17; male: n = 9; female: n = 8). ( F ) No altered NO-GC-β1 expression between IRAG1-KO (total: n = 22; male: n = 11; female: n = 11) and IRAG1-WT mice (total: n = 23; male: n = 12; female: n = 11). ( G ) Statistical analysis of the expression of investigated proteins ( A – F ). The mean ± SEM is shown in the graphs, and significant differences are shown by (*) ( p < 0.05), (**) ( p < 0.01) and (***) ( p < 0.001). Images of total protein (TP) were shown in .
Anti Ip3r, supplied by Wanleibio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
anti-ip3r - by Bioz Stars, 2026-02
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goat anti-ip3r antibodies gt328  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare goat anti-ip3r antibodies gt328
The expression of several cGMP/PKGI signaling proteins in colons from IRAG1-KO (KO) and IRAG1-WT (WT) mice. Protein expression of IRAG1-KO mice and their WT littermates (respective age: 15 weeks) were analyzed by western blot ( A – F ) and normalized to total protein (TP) for quantification ( G ). ( A ) Representative Western blot of IRAG1 in colons of male and female IRAG1-WT (total: n = 18; male: n = 10; female: n = 8) showed no differences between the sexes and no IRAG1 protein was found in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11). ( B ) Western blot of IP 3 R-I showed a significant decrease in its expression in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared to WT littermates (total: n = 21; male: n = 11; female: n = 10). ( C ) The tremendous reduction of PKGIβ protein in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared to IRAG1-WT mice (total: n = 24; male: n = 12; female: n = 12). ( D ) PKGIα expression was not altered between IRAG1-KO (total: n = 20; male: n = 10; female: n = 10) and IRAG1-WT mice (total: n = 22; male: n = 11; female: n = 11). ( E ) Reduced IP 3 R-III expression in IRAG1-KO mice (total: n = 21; male: n = 10; female: n = 11) in relation to IRAG1-WT mice (total: n = 17; male: n = 9; female: n = 8). ( F ) No altered NO-GC-β1 expression between IRAG1-KO (total: n = 22; male: n = 11; female: n = 11) and IRAG1-WT mice (total: n = 23; male: n = 12; female: n = 11). ( G ) Statistical analysis of the expression of investigated proteins ( A – F ). The mean ± SEM is shown in the graphs, and significant differences are shown by (*) ( p < 0.05), (**) ( p < 0.01) and (***) ( p < 0.001). Images of total protein (TP) were shown in .
Goat Anti Ip3r Antibodies Gt328, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti-ip3r antibodies gt328/product/Johns Hopkins HealthCare
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Image Search Results


The expression of several cGMP/PKGI signaling proteins in colons from IRAG1-KO (KO) and IRAG1-WT (WT) mice. Protein expression of IRAG1-KO mice and their WT littermates (respective age: 15 weeks) were analyzed by western blot ( A – F ) and normalized to total protein (TP) for quantification ( G ). ( A ) Representative Western blot of IRAG1 in colons of male and female IRAG1-WT (total: n = 18; male: n = 10; female: n = 8) showed no differences between the sexes and no IRAG1 protein was found in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11). ( B ) Western blot of IP 3 R-I showed a significant decrease in its expression in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared to WT littermates (total: n = 21; male: n = 11; female: n = 10). ( C ) The tremendous reduction of PKGIβ protein in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared to IRAG1-WT mice (total: n = 24; male: n = 12; female: n = 12). ( D ) PKGIα expression was not altered between IRAG1-KO (total: n = 20; male: n = 10; female: n = 10) and IRAG1-WT mice (total: n = 22; male: n = 11; female: n = 11). ( E ) Reduced IP 3 R-III expression in IRAG1-KO mice (total: n = 21; male: n = 10; female: n = 11) in relation to IRAG1-WT mice (total: n = 17; male: n = 9; female: n = 8). ( F ) No altered NO-GC-β1 expression between IRAG1-KO (total: n = 22; male: n = 11; female: n = 11) and IRAG1-WT mice (total: n = 23; male: n = 12; female: n = 11). ( G ) Statistical analysis of the expression of investigated proteins ( A – F ). The mean ± SEM is shown in the graphs, and significant differences are shown by (*) ( p < 0.05), (**) ( p < 0.01) and (***) ( p < 0.001). Images of total protein (TP) were shown in .

Journal: International Journal of Molecular Sciences

Article Title: Loss of PKGIβ/IRAG1 Signaling Causes Anemia-Associated Splenomegaly

doi: 10.3390/ijms22115458

Figure Lengend Snippet: The expression of several cGMP/PKGI signaling proteins in colons from IRAG1-KO (KO) and IRAG1-WT (WT) mice. Protein expression of IRAG1-KO mice and their WT littermates (respective age: 15 weeks) were analyzed by western blot ( A – F ) and normalized to total protein (TP) for quantification ( G ). ( A ) Representative Western blot of IRAG1 in colons of male and female IRAG1-WT (total: n = 18; male: n = 10; female: n = 8) showed no differences between the sexes and no IRAG1 protein was found in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11). ( B ) Western blot of IP 3 R-I showed a significant decrease in its expression in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared to WT littermates (total: n = 21; male: n = 11; female: n = 10). ( C ) The tremendous reduction of PKGIβ protein in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared to IRAG1-WT mice (total: n = 24; male: n = 12; female: n = 12). ( D ) PKGIα expression was not altered between IRAG1-KO (total: n = 20; male: n = 10; female: n = 10) and IRAG1-WT mice (total: n = 22; male: n = 11; female: n = 11). ( E ) Reduced IP 3 R-III expression in IRAG1-KO mice (total: n = 21; male: n = 10; female: n = 11) in relation to IRAG1-WT mice (total: n = 17; male: n = 9; female: n = 8). ( F ) No altered NO-GC-β1 expression between IRAG1-KO (total: n = 22; male: n = 11; female: n = 11) and IRAG1-WT mice (total: n = 23; male: n = 12; female: n = 11). ( G ) Statistical analysis of the expression of investigated proteins ( A – F ). The mean ± SEM is shown in the graphs, and significant differences are shown by (*) ( p < 0.05), (**) ( p < 0.01) and (***) ( p < 0.001). Images of total protein (TP) were shown in .

Article Snippet: The membrane was incubated in the primary antibody (anti-PKGIα (in-house production): 1:80 [ ]; anti-PKGIβ (in-house production): 1:200 [ ]; anti-FLC (abcam): 1:2000; anti-IP 3 R-I (Cell signaling): 1:250, anti-IP 3 R-III (BD Transduction Laboratories TM , BD Biosciences, San Jose, USA): 1:100; anti-IRAG1 (in-house production): 1:200 [ ]; anti-sGC-β-1 (NO-GC-β1) (Sigma-Aldrich ® , Taufkirchen, Germany): 1:500) overnight.

Techniques: Expressing, Western Blot

The expression of several cGMP/PKGI signaling proteins in spleens from IRAG1-KO (KO) and IRAG1-WT (WT) mice. Protein expression of IRAG1-KO mice and IRAG1-WT mice (respective age: 15 weeks) were analyzed by Western blot ( A – F ) and for quantification normalized to total protein (TP) ( G ). ( A ) Western blot of IRAG1 of male and female IRAG1-WT (total: n = 23; male: n = 11; female: n = 12) and IRAG1-KO mice (total: n = 24; male: n = 12; female: n = 12). ( B ) Increased expression of IP 3 R-I in IRAG1-KO mice (total: n = 22; male: n = 12; female: n = 10) compared to WT littermates (total: n = 22; male: n = 11; female: n = 11). ( C ) The expression of PKGIβ was reduced in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared with IRAG1-WT mice (total: n = 24; male: n = 12; female: n = 12). ( D ) PKGIα expression increased in IRAG1-KO mice (total: n = 24; male: n = 12; female: n = 12) in relation to their WT littermates (total: n = 22; male: n = 11; female: n = 11). ( E ): IRAG1-KO mice (total: n = 23; male: n = 12; female: n = 11) had an increase in IP 3 R-III expression than IRAG1-WT mice (total: n = 23; male: n = 11; female: n = 12). ( F ) Higher NO-GC-β1 expression was found in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) as in their WT littermates (total: n = 24; male: n = 11; female: n = 13). ( G ) Statistical analysis of the expression of investigated proteins ( A – F ). Mean ± SEM is shown in the graphs, and significant differences are shown by (*) ( p < 0.05), (**) ( p < 0.01) and (***) ( p < 0.001). Images of total protein (TP) were shown in .

Journal: International Journal of Molecular Sciences

Article Title: Loss of PKGIβ/IRAG1 Signaling Causes Anemia-Associated Splenomegaly

doi: 10.3390/ijms22115458

Figure Lengend Snippet: The expression of several cGMP/PKGI signaling proteins in spleens from IRAG1-KO (KO) and IRAG1-WT (WT) mice. Protein expression of IRAG1-KO mice and IRAG1-WT mice (respective age: 15 weeks) were analyzed by Western blot ( A – F ) and for quantification normalized to total protein (TP) ( G ). ( A ) Western blot of IRAG1 of male and female IRAG1-WT (total: n = 23; male: n = 11; female: n = 12) and IRAG1-KO mice (total: n = 24; male: n = 12; female: n = 12). ( B ) Increased expression of IP 3 R-I in IRAG1-KO mice (total: n = 22; male: n = 12; female: n = 10) compared to WT littermates (total: n = 22; male: n = 11; female: n = 11). ( C ) The expression of PKGIβ was reduced in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) compared with IRAG1-WT mice (total: n = 24; male: n = 12; female: n = 12). ( D ) PKGIα expression increased in IRAG1-KO mice (total: n = 24; male: n = 12; female: n = 12) in relation to their WT littermates (total: n = 22; male: n = 11; female: n = 11). ( E ): IRAG1-KO mice (total: n = 23; male: n = 12; female: n = 11) had an increase in IP 3 R-III expression than IRAG1-WT mice (total: n = 23; male: n = 11; female: n = 12). ( F ) Higher NO-GC-β1 expression was found in IRAG1-KO mice (total: n = 22; male: n = 11; female: n = 11) as in their WT littermates (total: n = 24; male: n = 11; female: n = 13). ( G ) Statistical analysis of the expression of investigated proteins ( A – F ). Mean ± SEM is shown in the graphs, and significant differences are shown by (*) ( p < 0.05), (**) ( p < 0.01) and (***) ( p < 0.001). Images of total protein (TP) were shown in .

Article Snippet: The membrane was incubated in the primary antibody (anti-PKGIα (in-house production): 1:80 [ ]; anti-PKGIβ (in-house production): 1:200 [ ]; anti-FLC (abcam): 1:2000; anti-IP 3 R-I (Cell signaling): 1:250, anti-IP 3 R-III (BD Transduction Laboratories TM , BD Biosciences, San Jose, USA): 1:100; anti-IRAG1 (in-house production): 1:200 [ ]; anti-sGC-β-1 (NO-GC-β1) (Sigma-Aldrich ® , Taufkirchen, Germany): 1:500) overnight.

Techniques: Expressing, Western Blot

The expression of several cGMP/PKGI signaling proteins in stomachs from IRAG1-KO (KO) and IRAG1-WT (WT) mice. Protein expression of IRAG1-KO mice and their WT littermates (respective age: 15 weeks) were analyzed by Western blot ( A – F ) and normalized to total protein (TP) ( G ). ( A ) Western blot of IRAG1 in IRAG1-WT (total: n = 22; male: n = 12; female: n = 10) and IRAG1-KO mice (total: n = 23; male: n = 11; female: n = 12). ( B ) Decreased expression of IP 3 R-I in IRAG1-KO mice (total: n = 22; male: n = 12; female: n = 10) compared to IRAG1-WT mice (total: n = 22; male: n = 12; female: n = 10). ( C ) The expression of PKGIβ was reduced in male and female IRAG1-KO mice (total: n = 23; male: n = 11; female: n = 12) in relation to WT littermates (total: n = 23; male: n = 12; female: n = 11). ( D ) Increased PKGIα expression was detected in IRAG1-KO mice (total: n = 21; male: n = 10; female: n = 11) towards their WT littermates (total: n = 24; male: n = 11; female: n = 13). ( E ) No difference in expression of IP 3 R-III between IRAG1-WT mice (total: n = 24; male: n = 11; female: n = 13) and IRAG1-KO mice (total: n = 22; male: n = 10; female: n = 12) were found. ( F ) Stomachs of IRAG1-KO mice (total: n = 23; male: n = 11; female: n = 12) indicated a decrease in NO-GC-β1 expression in relation to WT littermates (total: n = 25; male: n = 12; female: n = 13). ( G ) Statistical analysis of the expression of investigated proteins ( A – F ). The mean ± SEM is shown in the graphs, and significant differences are shown by (*) ( p < 0.05), (**) ( p < 0.01) and (***) ( p < 0.001). Images of total protein (TP) were shown in .

Journal: International Journal of Molecular Sciences

Article Title: Loss of PKGIβ/IRAG1 Signaling Causes Anemia-Associated Splenomegaly

doi: 10.3390/ijms22115458

Figure Lengend Snippet: The expression of several cGMP/PKGI signaling proteins in stomachs from IRAG1-KO (KO) and IRAG1-WT (WT) mice. Protein expression of IRAG1-KO mice and their WT littermates (respective age: 15 weeks) were analyzed by Western blot ( A – F ) and normalized to total protein (TP) ( G ). ( A ) Western blot of IRAG1 in IRAG1-WT (total: n = 22; male: n = 12; female: n = 10) and IRAG1-KO mice (total: n = 23; male: n = 11; female: n = 12). ( B ) Decreased expression of IP 3 R-I in IRAG1-KO mice (total: n = 22; male: n = 12; female: n = 10) compared to IRAG1-WT mice (total: n = 22; male: n = 12; female: n = 10). ( C ) The expression of PKGIβ was reduced in male and female IRAG1-KO mice (total: n = 23; male: n = 11; female: n = 12) in relation to WT littermates (total: n = 23; male: n = 12; female: n = 11). ( D ) Increased PKGIα expression was detected in IRAG1-KO mice (total: n = 21; male: n = 10; female: n = 11) towards their WT littermates (total: n = 24; male: n = 11; female: n = 13). ( E ) No difference in expression of IP 3 R-III between IRAG1-WT mice (total: n = 24; male: n = 11; female: n = 13) and IRAG1-KO mice (total: n = 22; male: n = 10; female: n = 12) were found. ( F ) Stomachs of IRAG1-KO mice (total: n = 23; male: n = 11; female: n = 12) indicated a decrease in NO-GC-β1 expression in relation to WT littermates (total: n = 25; male: n = 12; female: n = 13). ( G ) Statistical analysis of the expression of investigated proteins ( A – F ). The mean ± SEM is shown in the graphs, and significant differences are shown by (*) ( p < 0.05), (**) ( p < 0.01) and (***) ( p < 0.001). Images of total protein (TP) were shown in .

Article Snippet: The membrane was incubated in the primary antibody (anti-PKGIα (in-house production): 1:80 [ ]; anti-PKGIβ (in-house production): 1:200 [ ]; anti-FLC (abcam): 1:2000; anti-IP 3 R-I (Cell signaling): 1:250, anti-IP 3 R-III (BD Transduction Laboratories TM , BD Biosciences, San Jose, USA): 1:100; anti-IRAG1 (in-house production): 1:200 [ ]; anti-sGC-β-1 (NO-GC-β1) (Sigma-Aldrich ® , Taufkirchen, Germany): 1:500) overnight.

Techniques: Expressing, Western Blot